Share

Improved methods for controlled rapid cooling of cell suspensions.

Author(s) : MORRIS G. J., RICHENS H. E.

Type of article: Article

Summary

Simple, reproducible, methods of achieving rapid rates of cooling in the range 100 to 1000 °C/min are described for straws and cryovial. These methods use the direct contact of straws or cryovials with precooled granules or plates as the heat sink. Liquid nitrogen may be adsorbed into suitable material, for example activated charcoal, zeolites and molecular sieves as the matrix to achieve rapid cooling. Controllable rapid rates of cooling may also be attained by using non-adsorbents. The rate of cooling may be modified by changing the adsorbent material, the size of the adsorbent granules and the temperature of the adsorbent.

Details

  • Original title: Improved methods for controlled rapid cooling of cell suspensions.
  • Record ID : 2005-1500
  • Languages: English
  • Source: CryoLetters - vol. 25 - n. 4
  • Publication date: 2004/07

Links


See the source

Indexing