Partager

Document IIF

Mise au point d'un appareil servant à cryoconserver des échantillons cellulaires.

Development of an apparatus for cryopreservation cellular samples.

Numéro : pap. 019

Auteurs : BAUER R., LOEDER S., PIENKNY R., et al.

Résumé

Viability of cells after cryopreservation and re-vitalization is dependent on several factors. Of high importance are the seeding step and the cooling rate during cryopreservation. Spontaneous seeding without induced crystallisation leads to super-cooling effects and thereby to cell damage. Cells which are cooled too fast are destroyed due to the formation of intracellular ice and recrystallization effects when being re-vitalized. Cells, which are cooled too slowly, are exposed to solution effects and osmotic damage. Accordingly, apparatuses for cryopreserving cellular samples have to be constructed in a way to guarantee exact, simultaneous and reproducible seeding and subsequent cooling to avoid cooling rates that are too low or too high. In this publication, an apparatus is presented, which can fulfil the requirements for a defined cryopreservation process.

Documents disponibles

Format PDF

Pages : 5 p.

Disponible

  • Prix public

    20 €

  • Prix membre*

    Gratuit

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : Development of an apparatus for cryopreservation cellular samples.
  • Identifiant de la fiche : 30019506
  • Langues : Anglais
  • Source : 2nd IIR Workshop on cold applications in life sciences.
  • Date d'édition : 08/09/2016
  • DOI : http://dx.doi.org/10.18462/iir.cals.2016.0019

Liens


Voir d'autres communications du même compte rendu (25)
Voir le compte rendu de la conférence

Indexation