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IIR document

Development of an apparatus for cryopreservation cellular samples.

Number: pap. 019

Author(s) : BAUER R., LOEDER S., PIENKNY R., et al.

Summary

Viability of cells after cryopreservation and re-vitalization is dependent on several factors. Of high importance are the seeding step and the cooling rate during cryopreservation. Spontaneous seeding without induced crystallisation leads to super-cooling effects and thereby to cell damage. Cells which are cooled too fast are destroyed due to the formation of intracellular ice and recrystallization effects when being re-vitalized. Cells, which are cooled too slowly, are exposed to solution effects and osmotic damage. Accordingly, apparatuses for cryopreserving cellular samples have to be constructed in a way to guarantee exact, simultaneous and reproducible seeding and subsequent cooling to avoid cooling rates that are too low or too high. In this publication, an apparatus is presented, which can fulfil the requirements for a defined cryopreservation process.

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Pages: 5 p.

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Details

  • Original title: Development of an apparatus for cryopreservation cellular samples.
  • Record ID : 30019506
  • Languages: English
  • Source: 2nd IIR Workshop on cold applications in life sciences.
  • Publication date: 2016/09/08
  • DOI: http://dx.doi.org/10.18462/iir.cals.2016.0019

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