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Pregrowth-desiccation: a simple and efficient procedure for the cryopreservation of rice embryogenic suspension cells.

Author(s) : ZHANG Y. X., WANG J. H., BIAN H. W., et al.

Type of article: Article

Summary

Rice embryogenic suspension cells were cryopreserved by a pregrowth-desiccation procedure. Cells were precultured in liquid AA medium containing 0.175 mol/L sucrose for 3 days and then in liquid AA medium containing 0.4 mol/L sorbitol for 1 day. After air-drying for about 20 h to a water content of 10%, the cells were placed into cryotubes and quenched into liquid nitrogen. A survival rate of 96 plus or minus 6% (TTC reduction assay) or 100% (cell clump regrowth) was achieved. Cryostored cells revived very quickly during recovery culture and retained the ability to regenerate fertile plants. Air-drying, a method usually employed in cryopreservation of seeds of shoot tips, can thus be used as a simple and efficient procedure for the cryopreservation of precultured rice suspension cells.

Details

  • Original title: Pregrowth-desiccation: a simple and efficient procedure for the cryopreservation of rice embryogenic suspension cells.
  • Record ID : 2002-2147
  • Languages: English
  • Source: CryoLetters - vol. 22 - n. 4
  • Publication date: 2001/07
  • Document available for consultation in the library of the IIR headquarters only.

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