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Cryopreservation of stem cells in banks of umbilical blood and bone marrow.

[In Russian. / En russe.]

Author(s) : SMOLÂNINOV A. B., KOVAN'KO G. N., BAGAUTDINOV S. N., et al.

Type of article: Article

Summary

The nature of destruction of a cell during its freezing and the mechanism of prevention of this destruction using cryoprotectants has been considered. Special features of cryopreservation of founder cells (FC) and the technique of this process are described. A container for the storage of founder cells and methods of maintaining low temperatures during storage of FC: nitrogen vapour or liquid nitrogen chilling, and also mechanical chilling are described. Cryoprotectants for cryopreservation of FC: glycerol, dimethylsulfoxide, hydroxyethylstarch and other polymers are described in detail. Recommendations on chilling conditions of FC during their cryopreservation and heating before use given.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 2010-0279
  • Languages: Russian
  • Source: Vestnik Mezdunarodarnoj Akademii Holoda - n. 2
  • Publication date: 2009

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