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Cryopreservation of encapsulated sugarcane apices: effect of storage temperature and storage duration.

Author(s) : GONZÁLEZ-ARNAO M. T., URRA C., ENGELMANN F., et al.

Type of article: Article

Summary

Apices sampled on in vitro plantlets of sugarcane were cryopreserved using the encapsulation-dehydration technique, comprising pregrowth for 24 h in liquid medium with 0.75 M sucrose, desiccation to 20-25% moisture content (fresh weight basis) and rapid immersion in liquid nitrogen. Apices of one variety were stored for up to one year at -196, -70 or -25 °C, with or without previous transitory immersion in liquid nitrogen. Whereas the recovery percentage of apices stored at -196 °C remained high and constant throughout the experiment, recovery of apices conserved at -70 and -25 °C decreased, respectively. The recovery percentage of apices of four additional varieties remained stable throughout a 1 year storage period at -196 °C.

Details

  • Original title: Cryopreservation of encapsulated sugarcane apices: effect of storage temperature and storage duration.
  • Record ID : 2000-3210
  • Languages: English
  • Source: Cryo-Letters - vol. 20 - n. 6
  • Publication date: 1999/11
  • Document available for consultation in the library of the IIR headquarters only.

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