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L'expression de la protéine mitochondriale découplante des tubercules de pommes de terre, induite par la basse température et par la maturation.

Low temperature and aging-promoted expression of PUMP in potato tuber mitochondria.

Auteurs : NANTES I. L., FAGIAN M. M., CATISTI R., et al.

Type d'article : Article

Résumé

It was shown that the plant uncoupling mitochondrial protein (PUMP) present in potato tuber mitochondria was induced by aging at 28 °C and that this induction was strongly stimulated when the potato tubers were stored at low temperature (4 °C). PUMP activity was detected by the degree of linoleic acid (LA)-induced ATP-sensitive mitochondrial uncoupling measured as a function of the decrease in membrane potential. The PUMP content was evaluated by immunoblot analysis using polyclonal antibodies raised against potato PUMP that specifically detect a 32-kDa band. In agreement with the effect of LA on membrane potential, the content of the 32-kDa band increased during storage and was stimulated by low temperature. These results support the proposed role of PUMP in plant thermogenesis and possibly in fruit ripening and senescence.

Détails

  • Titre original : Low temperature and aging-promoted expression of PUMP in potato tuber mitochondria.
  • Identifiant de la fiche : 2001-0906
  • Langues : Anglais
  • Source : FEBS Lett. - vol. 457 - n. 1
  • Date d'édition : 1999

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