Share

Low temperature and aging-promoted expression of PUMP in potato tuber mitochondria.

Author(s) : NANTES I. L., FAGIAN M. M., CATISTI R., et al.

Type of article: Article

Summary

It was shown that the plant uncoupling mitochondrial protein (PUMP) present in potato tuber mitochondria was induced by aging at 28 °C and that this induction was strongly stimulated when the potato tubers were stored at low temperature (4 °C). PUMP activity was detected by the degree of linoleic acid (LA)-induced ATP-sensitive mitochondrial uncoupling measured as a function of the decrease in membrane potential. The PUMP content was evaluated by immunoblot analysis using polyclonal antibodies raised against potato PUMP that specifically detect a 32-kDa band. In agreement with the effect of LA on membrane potential, the content of the 32-kDa band increased during storage and was stimulated by low temperature. These results support the proposed role of PUMP in plant thermogenesis and possibly in fruit ripening and senescence.

Details

  • Original title: Low temperature and aging-promoted expression of PUMP in potato tuber mitochondria.
  • Record ID : 2001-0906
  • Languages: English
  • Source: FEBS Lett. - vol. 457 - n. 1
  • Publication date: 1999

Links


See the source

Indexing