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An improved dehydration protocol for cryopreservation of Brassica napus somatic embryos.

Protocole de déshydratation amélioré pour la cryoconservation des embryons somatiques de Brassica napus.

Auteurs : LI C., LOH C. S., SUN W. Q.

Type d'article : Article

Résumé

This study compared three predehydration methods for cryopreservation of somatic embryos of oilseed rape (Brassica napus L.). Slow-drying improved the survival after cryopreservation. The highest survival rate (55 plus or minus 3%) was observed with 75% relative humidity of air (RH). The best survival rate (81 plus or minus 3%) was obtained, using the stepwise slow-drying procedure in which RH was progressively lowered from 97 to 75% over a period of 6 days. The data suggest that the drying rate during dehydration greatly affects the survival of somatic embryos after cryopreservation.

Détails

  • Titre original : An improved dehydration protocol for cryopreservation of Brassica napus somatic embryos.
  • Identifiant de la fiche : 2000-2237
  • Langues : Anglais
  • Source : Cryo-Letters - vol. 20 - n. 4
  • Date d'édition : 07/1999
  • Disponible à la bibliothèque de l'IIF

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