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This study compared three predehydration methods for cryopreservation of somatic embryos of oilseed rape (Brassica napus L.). Slow-drying improved the survival after cryopreservation. The highest survival rate (55 plus or minus 3%) was observed with 75% relative humidity of air (RH). The best survival rate (81 plus or minus 3%) was obtained, using the stepwise slow-drying procedure in which RH was progressively lowered from 97 to 75% over a period of 6 days. The data suggest that the drying rate during dehydration greatly affects the survival of somatic embryos after cryopreservation.
Details
- Original title: An improved dehydration protocol for cryopreservation of Brassica napus somatic embryos.
- Record ID : 2000-2237
- Languages: English
- Source: Cryo-Letters - vol. 20 - n. 4
- Publication date: 1999/07
- Document available for consultation in the library of the IIR headquarters only.
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Cryopreservation of Arachis pintoi (leguminosae...
- Author(s) : REY H. Y., FALOCI M., MEDINA R., et al.
- Date : 2013/11
- Languages : English
- Source: CryoLetters - vol. 34 - n. 6
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Cryopreservation of apices isolated from garlic...
- Author(s) : MAKOWSKA Z., KELLER J., ENGELMANN F.
- Date : 1999/05
- Languages : English
- Source: Cryo-Letters - vol. 20 - n. 3
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Cryopreservation of Cosmos atrosanguineus shoot...
- Author(s) : WILKINSON T., WETTEN A., FAY M. F.
- Date : 1998/09
- Languages : English
- Source: Cryo-Letters - vol. 19 - n. 5
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Pregrowth-desiccation: a simple and efficient p...
- Author(s) : ZHANG Y. X., WANG J. H., BIAN H. W., et al.
- Date : 2001/07
- Languages : English
- Source: CryoLetters - vol. 22 - n. 4
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Cryopreservation of cultured plant cells and me...
- Author(s) : SAKAI A.
- Date : 1996
- Languages : Japanese
- Source: Seminar "Adaptation of plants to low temperatures: science and practice".
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