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Teneur en polyamines et en acides gras des méristèmes de bananes précultivés dans un milieu contenant du saccharose : corrélation avec le taux de survie après cryoconservation.

Polyamines and fatty acids in sucrose precultured banana meristems and correlation with survival rate after cryopreservation.

Auteurs : RAMON M., GEUNS J. M. C., SWENNEN R., et al.

Type d'article : Article

Résumé

Polyamines and fatty acids were studied in proliferating meristem cultures of 3 banana cultivars with high (Cachaco), medium (Williams Bronze free) and low (Mbwazirume) survival rates after cryopreservation. A 2-week preculture on medium containing 0.4 M sucrose which is essential to obtain survival after cryopreservation resulted in increased polyamine, especially putrescine levels. This increase in putrescine content was positively correlated with the survival rate after simple freezing or after vitrification. The total fatty acid content also increased after a 0.4 M sucrose pretreatment. However, only the ratio of unsaturated/saturated fatty acids correlated positively with the survival rate after cryopreservation. This is the first report showing a correlation of both putrescine increase and level of unsaturation of membrane lipids after sucrose treatment with survival rate after cryopreservation.

Détails

  • Titre original : Polyamines and fatty acids in sucrose precultured banana meristems and correlation with survival rate after cryopreservation.
  • Identifiant de la fiche : 2003-3031
  • Langues : Anglais
  • Source : CryoLetters - vol. 23 - n. 6
  • Date d'édition : 11/2002
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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