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Does lipid profile explain chilling sensitivity and membrane lipid phase transition of spermatozoa and oocytes?

Author(s) : ARAV A., PEARL M., ZERON Y.

Type of article: Article

Summary

Ram, fowl and bee spermatozoa, and oocytes of cows and zebrafish were used to study lipid membrane profiles, chilling sensitivity and lipid-phase transitions. The integrity of the membranes was determined by carboxyfluorescein diacetate (cFDA) staining following exposure for 15 minutes to low temperatures. Lipid phase transition (LPT) and membrane fluidity, which were evaluated by Fourier transform infrared (FTIR) microscopy, and fluorescence polarization, showed phase transitions at the same temperatures as caused damage (between 30 and 12 °C). These results suggest a close relationship among cold susceptibility, lipid phase transition and lipids profile in animal gametes.

Details

  • Original title: Does lipid profile explain chilling sensitivity and membrane lipid phase transition of spermatozoa and oocytes?
  • Record ID : 2001-1095
  • Languages: English
  • Source: CryoLetters - vol. 21 - n. 3
  • Publication date: 2000/06
  • Document available for consultation in the library of the IIR headquarters only.

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