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Charger les globules rouges en tréhalose : un pas vers la biostabilisation.

Loading red blood cells with trehalose: a step towards biostabilization.

Auteurs : SATPATHY G. R., TÖRÖK Z., BALI R., et al.

Type d'article : Article

Résumé

A method for freeze-drying red blood cells (RBCs) while maintaining a high degree of viability has important implications in blood transfusion and clinical medicine. The disaccharide trehalose, found in animals capable of surviving dehydration can aid in this process. As a first step toward RBC preservation, the author present a method for loading RBCs with trehalose. The method is based on the thermal properties of the RBC plasma membranes and provides efficient uptake of the sugar at 37 °C over 7 h. The data show that RBCs can be loaded with trehalose through a combination of osmotic imbalance and the phospholipid phase transition, resulting in intracellular trehalose concentrations of about 40 mM. During the loading period, the levels of ATP and 2,3-DPG are maintained close to the levels of fresh RBCs. Increasing the membrane fluidity through the use of a benzyl alcohol results in a higher concentration of intracellular trehalose. Osmotic fragility data show that trehalose exerts osmotic protection on RBCs. Flow cytometry data demonstrate that incubation of RBCs in a hypertonic trehalose solution results in a fraction of cells with different complexity and that it can be removed by washing and resuspending the RBCs in an iso-osmotic medium. The data provide an important first step in long-term preservation of RBCs.

Détails

  • Titre original : Loading red blood cells with trehalose: a step towards biostabilization.
  • Identifiant de la fiche : 2005-1499
  • Langues : Anglais
  • Source : Cryobiology - vol. 49 - n. 2
  • Date d'édition : 10/2004

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