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Cryobank of plant genetic resources in Russian Academy of Sciences.

Author(s) : POPOV A. S., POPOVA E. V., NIKISHINA T. V., et al.

Type of article: Article, IJR article

Summary

Cryopreservation is the most reliable method for long-term storage of plant genetic resources. A review of cell injury by ice crystals and dehydration during a freeze-thaw cycle is given. For successful regeneration of plants and cultures after cryopreservation of their cells, the development of reliable cryopreservation procedure is required including preliminary cultivation, treatment by cryoprotectors, freezing by different methods, thawing and recultivation. Up to now, 27 cell lines successfully resumed their growth after storage in liquid nitrogen and preserved their specific features and biosynthetic potential. Besides, shoot tips of 40 cultivars of potato, rose, strawberry and raspberry regenerated plants both in vitro and in vivo after cryopreservation. The longest storage duration was 25 years. Today, 24 cell strains of rare medicinal plants, shoot tips of seven cultivars of strawberry and raspberry and seeds of 250 endangered plant species collected over all Russian territory are continuously stored in liquid nitrogen.

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Format PDF

Pages: 403-410

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Details

  • Original title: Cryobank of plant genetic resources in Russian Academy of Sciences.
  • Record ID : 2006-1520
  • Languages: English
  • Source: International Journal of Refrigeration - Revue Internationale du Froid - vol. 29 - n. 3
  • Publication date: 2006/05

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