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Cryoconservation des hépatocytes humains grâce à des agents cryoprotecteurs à base d'alcool et de sucre.

[In Chinese. / En chinois.]

Auteurs : YANG B., SHEN L., HUANG D., et al.

Type d'article : Article

Résumé

Different types of sugar alcohol areusually used as cryoprotective agants (CPAs) to preserve human hepatocytes. In this study, a new type of sugar alcohol based CPAs with low toxicity was explored by replacing Me(2)SO with D-sorbic alcohol, xylosic alcohol and maltol. The Me(2)SO concentration in CPAs was reduced to 5%. Human hepatocytes attached to plastic cover slips were treated with the above CPAs and then placed in the fridge at -80°C for two weeks. The survival rate, 24h cell detachment and cell morphology were tested and compared with the results using 10%Me(2)SO as CPAs. The results show that 5% Me(2)SO with D-sorbic alcohol gives the best results. It is concluded that the new CPAs are effective to human hepatocytes cryopreservation.

Documents disponibles

Format PDF

Pages : 62-67

Disponible

  • Prix public

    20 €

  • Prix membre*

    15 €

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : [In Chinese. / En chinois.]
  • Identifiant de la fiche : 30005218
  • Langues : Chinois
  • Source : Journal of Refrigeration - vol. 33 - n. 145
  • Date d'édition : 06/2012

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