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Cryopreservation of human hepatocytes by sugar-alcohol based CPAs.

[In Chinese. / En chinois.]

Author(s) : YANG B., SHEN L., HUANG D., et al.

Type of article: Article

Summary

Different types of sugar alcohol areusually used as cryoprotective agants (CPAs) to preserve human hepatocytes. In this study, a new type of sugar alcohol based CPAs with low toxicity was explored by replacing Me(2)SO with D-sorbic alcohol, xylosic alcohol and maltol. The Me(2)SO concentration in CPAs was reduced to 5%. Human hepatocytes attached to plastic cover slips were treated with the above CPAs and then placed in the fridge at -80°C for two weeks. The survival rate, 24h cell detachment and cell morphology were tested and compared with the results using 10%Me(2)SO as CPAs. The results show that 5% Me(2)SO with D-sorbic alcohol gives the best results. It is concluded that the new CPAs are effective to human hepatocytes cryopreservation.

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Pages: 62-67

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Details

  • Original title: [In Chinese. / En chinois.]
  • Record ID : 30005218
  • Languages: Chinese
  • Source: Journal of Refrigeration - vol. 33 - n. 145
  • Publication date: 2012/06

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