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Performance thermique des capillaires de quartz pour la vitrification.

Thermal performance of quartz capillaries for vitrification.

Auteurs : RISCO R., ELMOAZZEN H., DOUGHTY M., et al.

Type d'article : Article

Résumé

In this paper the authors report the thermal behaviour of a new approach for vitrification. Thermal performance of traditional open pulled straws is compared with a new technique based on the combined use of quartz capillaries with slush nitrogen. This new method of vitrification achieved ultrafast cooling rates of 250,000°C/min. As a result, a much lower concentration of cryoprotectant was needed to reach vitrification. In fact, a cryoprotectant solution typically used in oocyte slow freezing protocols was shown to remain transparent after cooling to liquid nitrogen temperatures indicating apparent "vitrification". This approach offers a new and very promising technique for vitrification of cells using low levels of cryoprotectants. [Reprinted with permission from Elsevier. Copyright, 2007].

Détails

  • Titre original : Thermal performance of quartz capillaries for vitrification.
  • Identifiant de la fiche : 2008-0940
  • Langues : Anglais
  • Source : Cryobiology - vol. 55 - n. 3
  • Date d'édition : 12/2007

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