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Thermal performance of quartz capillaries for vitrification.

Author(s) : RISCO R., ELMOAZZEN H., DOUGHTY M., et al.

Type of article: Article

Summary

In this paper the authors report the thermal behaviour of a new approach for vitrification. Thermal performance of traditional open pulled straws is compared with a new technique based on the combined use of quartz capillaries with slush nitrogen. This new method of vitrification achieved ultrafast cooling rates of 250,000°C/min. As a result, a much lower concentration of cryoprotectant was needed to reach vitrification. In fact, a cryoprotectant solution typically used in oocyte slow freezing protocols was shown to remain transparent after cooling to liquid nitrogen temperatures indicating apparent "vitrification". This approach offers a new and very promising technique for vitrification of cells using low levels of cryoprotectants. [Reprinted with permission from Elsevier. Copyright, 2007].

Details

  • Original title: Thermal performance of quartz capillaries for vitrification.
  • Record ID : 2008-0940
  • Languages: English
  • Source: Cryobiology - vol. 55 - n. 3
  • Publication date: 2007/12

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