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Composition and behaviour of head membrane lipids of fresh and cryopreserved boar sperm.

Author(s) : BUHR M. M., CURTIS E. F., KAKUDA N. S.

Type of article: Article

Summary

Head plasma membranes were isolated from fresh or cryopreserved ejaculated boar spermatazoa and the lipids were extracted for determination of lipid fluidity and for compositional analysis. Fluidity of liposomes declined significantly during controlled-rate cooling for all samples. Gel lipids from cryopreserved cells lost fluidity much more rapidly than lipids from fresh membranes. Fluidity increased during subsequent rewarming (5 to 50 deg C), again at a slower rate for lipids from fresh cells. Calcium altered the fluidity characteristics of membrane lipids from fresh but not cryopreserved sperm.

Details

  • Original title: Composition and behaviour of head membrane lipids of fresh and cryopreserved boar sperm.
  • Record ID : 1995-0576
  • Languages: English
  • Source: Cryobiology - vol. 31 - n. 3
  • Publication date: 1994/06

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