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Cryopreservation of mature bovine oocytes by vitrification in straws.

Author(s) : OTOI T., YAMAMOTO K., KOYAMA N., TACHIKAWA S., SUZUKI T.

Type of article: Article

Summary

Experiments were conducted to determine optimal conditions for vitrification of in vitro matured bovine oocytes in straws. The rates of morphological survival and cleavage of oocytes vitrified by the three-step addition procedure were higher than those vitrified by the one-step addition procedure. The survival rates of oocytes vitrified in 20 and 30% ethylene glycol (EG) were lower than those in 40 and 50% EG. The rates of cleavage and development to blastocysts of oocytes vitrified in 40% EG were the highest. The highest development rate (10%) to blastocysts was observed when oocytes were exposed to sucrose for 5 minutes. These data demonstrate that improvements to the concentration of cryoprotectant and addition procedures have critical effects on the development competence of oocytes vitrified in straws.

Details

  • Original title: Cryopreservation of mature bovine oocytes by vitrification in straws.
  • Record ID : 1999-3885
  • Languages: English
  • Source: Cryobiology - vol. 37 - n. 1
  • Publication date: 1998/08

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