ShareCryopreservation of totipotent nuclei from honeybee (Apis mellifera) embryos by rapid freezing.
Author(s) : YU R., HAGEN A., OMHOLT S. W.
Type of article: Article
Summary
The method reported is without the addition of a specific cryoprotectant or other additive. By making feasible long-term storage of ooplasm biopsied from preblastoderm embryos, the method could become a key element for the development of an efficient and practical procedure for cloning and other genetic studies of honeybees. The chimeric frequency (11.5%) was 85% of the frequency achieved when using noncryopreserved nuclei and the same transplantation protocol.
Details
- Original title: Cryopreservation of totipotent nuclei from honeybee (Apis mellifera) embryos by rapid freezing.
- Record ID : 1998-3896
- Languages: English
- Source: Cryobiology - vol. 35 - n. 1
- Publication date: 1997/08
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Insect; Cryobiology; Embryo; Cryopreservation
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