Development of in vitro matured bovine oocytes activated with low temperature.
Author(s) : OTOI T., YAMAMOTO K., KOYAMA N., TACHIKAWA S., SUZUKI T.
Type of article: Article
Summary
Bovine follicular oocytes cultured in vitro for 24 hours were activated by exposure to low temperature (1 deg C) and the developmental potential of the oocytes was then assessed. About 10% of oocytes were parthenogenetically activated by exposure to low temperature. After exposure to low temperature and subsequent treatment with cycloheximide and cytochalasin D, 44.7 and 3.3% of activated oocytes cleaved and developed to blastocysts, respectively.
Details
- Original title: Development of in vitro matured bovine oocytes activated with low temperature.
- Record ID : 1997-1136
- Languages: English
- Source: Cryo-Letters - vol. 17 - n. 3
- Publication date: 1996/05
- Document available for consultation in the library of the IIR headquarters only.
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Ovule; Cryobiology; Bovine; Temperature; Survival; Development; Additive
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