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Flow cytometric evaluation of mitochondrial activity and membrane integrity in fresh and cryopreserved rainbow trout (Oncorhynchus mykiss) spermatozoa.

Author(s) : OGIER DE BAULNY B., LE VERN Y., KERBOEUF D., MAISSE G.

Type of article: Article

Summary

After cryopreservation, the percentage of spermatozoa with damaged membrane varied between 55 and 90%, while only a few cells (between 0.5 and 17%) displayed both an undamaged membrane and an intact mitochondrion. The plasma membrane was better protected with 10 % DMSO, while methanol and propylene glycol showed toxic effects. The best cryoprotectant for the mitochondrion was DMSO at a concentration of 5 or 10%. These results are in agreement with fertility rates generally observed. Frozen-thawed spermatozoa lost between 50 and 90% of their initial adenosine 5'-triphosphate (ATP) content, and the origin of this loss is discussed.

Details

  • Original title: Flow cytometric evaluation of mitochondrial activity and membrane integrity in fresh and cryopreserved rainbow trout (Oncorhynchus mykiss) spermatozoa.
  • Record ID : 1998-1297
  • Languages: English
  • Source: Cryobiology - vol. 34 - n. 2
  • Publication date: 1997/03

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