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Membrane integrity, mitochondrial activity, ATP content, and motility of the European catfish (Silurus glanis) testicular spermatozoa after freezing with different cryoprotectants.

Author(s) : OGIER DE BAULNY B., LABBE C., MAISSE G.

Type of article: Article

Summary

The extent of cellular damage was investigated after freeze-thawing of European catfish testicular sperm with various cryoprotectants. The best protection was given by dimethylacetamide (10 and 15%) in a sucrose solution. Under these conditions, the percentage of cells with an intact membrane was high (90%), and the protection of the activity of the mitochondria was medium (47%). It was shown that the addition of dimethylacetamide largely increased the ATP content of the spermatozoa. It is suggested that this phenomenon is a decisive factor for the freezing resistance of European catfish testicular spermatozoa in the presence of dimethylacetamide (60% motility after thawing versus 90% before freezing).

Details

  • Original title: Membrane integrity, mitochondrial activity, ATP content, and motility of the European catfish (Silurus glanis) testicular spermatozoa after freezing with different cryoprotectants.
  • Record ID : 2001-0549
  • Languages: English
  • Source: Cryobiology - vol. 39 - n. 2
  • Publication date: 1999/09

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