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Preparation of fibrin glue from human plasma by double cryoprecipitation method.

[In Japanese. / En japonais.]

Author(s) : AKINO M., SEGAWA K., CHIBA S., TAKAHASHI T. A., SEKIGUCHI S.

Type of article: Article

Summary

The authors prepared fibrin glue from fibrinogen concentrated by cryoprecipitation of human plasma. Plasma collected from whole blood or by apheresis was frozen in a deep freezer at -80 deg C and stored for 1 to 18 hours and thawed rapidly in a waterbath or slowly in a refrigerator at 4 deg C. The thawed plasma was refrozen and thawed once more. The second cryoprecipitation increased the fibrinogen yield. The authors also developed a method to measure the adhesive strength of fibrin glue obtained by this double cryoprecipitation method.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 1994-2558
  • Languages: Japanese
  • Source: Cryomedicine - vol. 19 - n. 1
  • Publication date: 1993/03
  • Document available for consultation in the library of the IIR headquarters only.

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