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Simultaneous preservation of orchid seed and its fungal symbiont using encapsulation-dehydration is dependent on moisture content and storage temperature.

Author(s) : WOOD C. B., PRITCHARD H. W., MILLER A. P.

Type of article: Article

Summary

Seeds of Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid) were encapsulated in alginate beads with hyphae of the basidomycete fungus Ceratobasidium cornigerum. Pretreatment of beads for 18 h with sucrose at an optimum concentration of 0.75 M decreased the desiccation rate in a flow of sterile air (c. 23 °C, 30% RH) and increased seed and fungal survival after up to 16 h drying. The results indicate opportunities for the use of simultaneous cryopreservation as a conservation tool for diverse taxa.

Details

  • Original title: Simultaneous preservation of orchid seed and its fungal symbiont using encapsulation-dehydration is dependent on moisture content and storage temperature.
  • Record ID : 2001-1458
  • Languages: English
  • Source: CryoLetters - vol. 21 - n. 2
  • Publication date: 2000/03
  • Document available for consultation in the library of the IIR headquarters only.

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