ShareUltrarapid freezing of rat embryos with rapid dilution of permeable cryoprotectants.
Author(s) : ISACHENKO V. V., ISACHENKO E. F., OSTASHKO F. I., GRISHCHENKO V. I.
Type of article: Article
Summary
The highest rates of embryo development followed exposure of the embryos to 30% glycerol + 0.7 mol sucrose at 4 deg C for 2-3 minutes (95-84%) or at 24 deg C for 0.5-3 minutes (90-88%). The results indicate that it is possible to develop a method for the ultrarapid freezing of mammalian embryos that does not require dilution of permeable cryoprotectants in a hypertonic sucrose solution.
Details
- Original title: Ultrarapid freezing of rat embryos with rapid dilution of permeable cryoprotectants.
- Record ID : 1998-1298
- Languages: English
- Source: Cryobiology - vol. 34 - n. 2
- Publication date: 1997/03
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Rat; Embryo; Cryopreservation
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Cryoprotective effect of polyols on rat embryos...
- Author(s) : UTSUMI K., HOCHI S., IRITANI A.
- Date : 1992/06
- Languages : English
- Source: Cryobiology - vol. 29 - n. 3
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Simple and rapid cryopreservation of rat embryo...
- Author(s) : OHBOSHI S., FUJIHARA N.
- Date : 1992/03
- Languages : English
- Source: Low Temperature Medicine - vol. 18 - n. 1
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Antigen presenting function of cryopreserved ra...
- Author(s) : MARINOVA-MUTAFCHIEVA L., NERONOV A. J.
- Date : 1994/09
- Languages : English
- Source: Cryo-Letters - vol. 15 - n. 5
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CRYOPRESERVATION OF RAT BLASTOCYSTS BY VITRIFIC...
- Author(s) : KONO T., SUZUKI O., TSUNODA Y.
- Date : 1988
- Languages : English
- Source: Cryobiology - vol. 25 - n. 2
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A study on cryoprotectant solution suitable for...
- Author(s) : ETO T., TAKAHASHI R., KAMISAKO T., et al.
- Date : 2014/02
- Languages : English
- Source: Cryobiology - vol. 68 - n. 1
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