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Cryoprotective effect of polyols on rat embryos during two-step freezing.

Author(s) : UTSUMI K., HOCHI S., IRITANI A.

Type of article: Article

Summary

The cryoprotective effect of polyols on rat embryos was measured after two-step freezing, and the mechanism of action of polyols on embryo survival was examined. Rat embryos frozen in solution of polyol by two-step methods at the morula stage showed higher survival than that obtained using DMSO. The highest survival was obtained with adonitol. The findings exposed suggest that one cryoprotective action of polyols is that the hydroxyl group act both on the cell surface and the cytoplasm to stabilize the bound water on the embryonic membrane and that the length of the C-chain determines the permeability of the membrane to the additive.

Details

  • Original title: Cryoprotective effect of polyols on rat embryos during two-step freezing.
  • Record ID : 1993-1133
  • Languages: English
  • Source: Cryobiology - vol. 29 - n. 3
  • Publication date: 1992/06

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