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Zygotic and nucellar embryo survival following dehydration/cryopreservation of Citrus intact seeds.

Author(s) : LAMBARDI M., DE CARLO A., BIRICOLTI S., et al.

Type of article: Article

Summary

A cryopreservation procedure by dehydration and direct immersion in liquid nitrogen was developed for seeds of four polyembryonic Citrus species, and the sexual or nucellar origin of the recovered seedlings was investigated. Seeds of three species were desiccated in a sterile air flow to 16% (C. sinensis) or 10% (C. aurantium and C. limon) moisture content with a negligible reduction in germination levels. The germinated seed was always observed. However, all four species benefited from desiccation in terms of protection during immersion in liquid nitrogen, with C. sinensis and C. aurantium showing the greatest survival (93% germination) after cryopreservation. Analysis of seedlings recovered from cryopreserved seeds showed that the dehydration/cryopreservation procedure promotes the germination of zygotic embryos and reduces the number of apomictic seedlings per seed.

Details

  • Original title: Zygotic and nucellar embryo survival following dehydration/cryopreservation of Citrus intact seeds.
  • Record ID : 2005-0438
  • Languages: English
  • Source: CryoLetters - vol. 25 - n. 2
  • Publication date: 2004/03

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