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Cryopreservation of Citrus madurensis embryonic axes by encapsulation-dehydration.

Author(s) : CHO E. G., HOR Y. L., KIM H. H., et al.

Type of article: Article

Summary

The authors demonstrated that Citrus madurensis embryonic axes can withstand cryopreservation using the encapsulation-dehydration technique. Up to 57.5% survival was achieved using a standard encapsulation-dehydration protocol, which included pregrowth of encapsulated axes for 16 h in medium containing 0.8 M sucrose + 1 M glycerol, desiccation of beads to around 30% moisture content followed by rapid freezing. A slightly higher survival percentage (65%) was obtained using a modified encapsulation-dehydration protocol, which included pretreatment of axes with 2 M glycerol + 0.6 M sucrose for 1 h, concomitantly with their encapsulation in 3% calcium alginate beads, followed by desiccation of the beads to around 30% moisture content.

Details

  • Original title: Cryopreservation of Citrus madurensis embryonic axes by encapsulation-dehydration.
  • Record ID : 2003-3027
  • Languages: English
  • Source: CryoLetters - vol. 23 - n. 5
  • Publication date: 2002/09
  • Document available for consultation in the library of the IIR headquarters only.

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