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ALTERATION OF SEMINAL PROTEINS DURING FREEZE-DRYING OF BOVINE SEMEN.

Author(s) : JEYENDRAN R. S., HUNTER A. G., GRAHAM E. F.

Type of article: Article

Summary

BOVINE SEMEN IN TEST-YOLK EXTENDER WAS FROZEN, FREEZE-DRIED TO 50, 25, 12, 6, AND LESS THAN 2% RESIDUAL MOISTURE, AND STORED AT 77 K (-196 DEG C). THE FREEZE-DRIED SEMEN WAS REHYDRATED, SAMPLED FOR PROTEIN ANALYSIS, AND USED TO INSEMINATE CATTLE. IMMUNODIFFUSION DATA WITH ANTISERA AGAINST SPERMATOZOA AND SEMINAL PLASMA REVEALED NO DIFFERENCE IN FORMATION OF PRECIPITIN LINES IF RESIDUAL MOISTURE WAS AT LEAST 6%. HOWEVER, SEMEN FREEZE-DRIED TO 2% RESIDUAL MOISTURE WAS MODIFIED ANTIGENICALLY AS CERTAIN PRECIPITIN LINES WERE LOST, NEW LINES APPEARED, AND CONCENTRATION OF OTHER SEMINAL ANTIGENS DECREASED.

Details

  • Original title: ALTERATION OF SEMINAL PROTEINS DURING FREEZE-DRYING OF BOVINE SEMEN.
  • Record ID : 1984-0396
  • Languages: English
  • Publication date: 1983/04
  • Source: Source: J. Dairy Sci.
    vol. 66; n. 4; 887-891; 5 fig.; 21 ref.
  • Document available for consultation in the library of the IIR headquarters only.

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