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CONTRIBUTIONS OF THE FREEZE-FRACTURE TECHNIQUE TO THE STUDY OF BIOLOGICAL MEMBRANES.

[In Italian. / En italien.]

Author(s) : ARANCIA G.

Type of article: Article

Summary

THE FREEZE-FRACTURE TECHNIQUE IS VERY HELPFUL IN THE STUDY OF BIOLOGICAL MEMBRANE ULTRASTRUCTURE. THIS PREPARATION METHOD FOR THE ELECTRON MICROSCOPY ALLOWS TO OBTAIN A REPLICA OF THE FRACTURE SURFACE OF THE SAMPLE PREVIOUSLY FROZEN AT VERY LOW TEMPERATURE. SINCE THE FRACTURE OCCURS THROUGH PREFERENTIAL PLANES AS THE HYDROPHOBIC CENTRES OF MEMBRANES, FREEZE-FRACTURING IS PARTICULARLY SUITABLE FOR THE DIRECT VISUALIZATION AND FOR THE STUDY OF THE MOLECULAR ORGANIZATION OF THE MEMBRANE COMPONENTS. IN THE PRESENT REVIEW, THE EVOLUTION OF THE STRUCTURAL MODELS OF BIOLOGICAL MEMBRANES IS FIRSTLY REPORTED. THEN THE DIFFERENT STEPS OF THE FREEZE-FRACTURE TECHNIQUE AND THEIR POSSIBLE EFFECTS ON MEMBRANE STRUCTURES ARE DESCRIBED.

Details

  • Original title: [In Italian. / En italien.]
  • Record ID : 1985-1746
  • Languages: Italian
  • Source: Ann. Ist. super. Sanità - vol. 19 - n. 2-3
  • Publication date: 1983
  • Document available for consultation in the library of the IIR headquarters only.

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