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Cooling rate measurement and system optimization for cryotop device.

[In Chinese. / En chinois.]

Author(s) : LI W., ZHOU X. L., DAI J. J., et al.

Type of article: Article

Summary

Cryotop is one of the best effective methods for oocytes cryopreservation. When Cryotop is inserted into liquid nitrogen. the cooling speed is extremely high, which is difficult to measure by a conventional method. In this study, digital oscilloscopes and type T thermocouples of 25 micro-m diameter were employed to establish a high-speed temperature measurement system. The cooling rates of Cryotop device at different carrier material, thickness and cold source temperature were measured. The results showed that when 60 micro-m copper was used as a carrier and slush nitrogen was used as a cold source, the cooling rate were increased. It could reach (37130 ± 1336) K/min, which was twice higher than that of commercially available Cryotop ( (11982 ± 1936) K/min).

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Pages: 95-98

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