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Cryobiology for transplant surgeons.

[In Japanese. / En japonais.]

Author(s) : SUMIDA S.

Type of article: Article

Summary

The tissue and organ to be frozen and thawed are usually irregular and bulky. The grafts will not be cooled in a destined and constant rate from the surface of the core. Every graft consists of different kinds of cells having different sensitivity for sub-zero temperatures. When the grafts protected with cryophylactic solution are solidified at the temperature of solidification or of glass transition (usually at -120 deg C or lower), the graft will solidify in a hybrid form of solid with considerable components in the glassy (vitrified) phase, and with components in the frozen state. When those grafts are warmed, the glassy parts will be abruptly devitrified and crystallized, resulting in volume expansion to crack the graft. Therefore, the frozen grafts should be stored above, at a constant temperature to avoid recrystallization.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 1997-3059
  • Languages: Japanese
  • Source: Low Temperature Medicine - vol. 22 - n. 3
  • Publication date: 1996/09
  • Document available for consultation in the library of the IIR headquarters only.

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