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Cryopreservation of Fraxinus excelsior L. zygotic embryos.

Author(s) : BREARLY J., HENSHAW G. G., DAVEY C., TAYLOR N. J., BLAKESLEY D.

Type of article: Article

Summary

Mature zygotic embryos of Ash were cryopreserved following a simple dehydration treatment. 63% germination was obtained when embryos were dried to a moisture content of 12-14% prior to rapid freezing in liquid nitrogen. Embryos transferred to Murashige and Skoog basal medium for 16-48 hours following excision required additional 0.4 M sucrose preculture to give some protection against freezing damage.

Details

  • Original title: Cryopreservation of Fraxinus excelsior L. zygotic embryos.
  • Record ID : 1996-1620
  • Languages: English
  • Source: Cryo-Letters - vol. 16 - n. 4
  • Publication date: 1995/07
  • Document available for consultation in the library of the IIR headquarters only.

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