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Cryopreservation of human brain tissue allowing timely production of viable adult human brain cells for autologous transplantation.

Author(s) : BRUNET J. F., PELLERIN L., MAGISTRETTI P., et al.

Type of article: Article

Summary

Autologous transplantation is an attractive approach to treat some neurological diseases. A major obstacle is the capacity to produce cells for transplantation at the appropriate time. The authors describe a cryopreservation procedure for adult human brain tissue allowing the generation of cells in vitro. Resections were dissected to separate white and grey matter. Fractions were frozen in a specific cryopreservation medium containing a selected serum and stored in liquid nitrogen. Tissue was thawed, cells were mechanically dissociated, expanded in culture and characterized by immunochemistry. Adult human brain tissue cryopreserved for up to two years was successfully used to generate brain cells that could be maintained in culture for up to 100 days. The procedure successfully enabled cryopreservation of adult human brain tissue that might facilitate future autologous transplantation strategies.

Details

  • Original title: Cryopreservation of human brain tissue allowing timely production of viable adult human brain cells for autologous transplantation.
  • Record ID : 2004-1975
  • Languages: English
  • Source: Cryobiology - vol. 47 - n. 2
  • Publication date: 2003/10

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