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Diffusion of dimethyl sulfoxide in tissue engineered collagen scaffolds visualized by computer tomography.

Author(s) : BERNEMANN I., MANUCHEHRABADI N., SPINDLER R., et al.

Type of article: Article

Summary

The authors cryopreserved chick islets using a new medium: a Dulbecco's modified minimum essential medium comprising Ham's F12 (1:1), fetal bovine serum (20%), dimethylsulfoxide (10%) and different concentrations (ranging from 50 to 500 microg/ml) of riboflavin and nicotinamide. Optimal recovery of viable islets was achieved using 250 microg/ml of riboflavin or nicotinamide. Chick islets are being investigated with a view to possible use in hypoglycemic drug screening.

Details

  • Original title: Diffusion of dimethyl sulfoxide in tissue engineered collagen scaffolds visualized by computer tomography.
  • Record ID : 30004662
  • Languages: English
  • Source: CryoLetters - vol. 31 - n. 6
  • Publication date: 2010/11

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