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Investigation of the influence of cell density of human fibroblasts cryopreserved inside collagen sponges at various cooling rates.

Author(s) : MATSUMURA Y., UJIHIRA M., NOGAWA S., et al.

Type of article: Article

Summary

In this study, human fibroblasts were cryopreserved inside a collagen matrix (a 20-mm diameter and 1-mm thickness collagen sponge) and several cooling rates were applied. The membrane integrity of the fibroblasts was evaluated using a trypan blue exclusion assay. High cell density was seen to adversely affect viability.

Details

  • Original title: Investigation of the influence of cell density of human fibroblasts cryopreserved inside collagen sponges at various cooling rates.
  • Record ID : 2008-1807
  • Languages: English
  • Source: Cryo-Letters - vol. 28 - n. 5
  • Publication date: 2007/09

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