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Effect of cryoprotectants on activity of selected enzymes in fish embryos.

Author(s) : ADAM M. M., RANA K. J., MCANDREW B. J.

Type of article: Article

Summary

Rosy barb (Puntius conchonius) and zebra fish (Brachydanio rerio) embryos at cleavage, epiboly, and closure of bastopore stages were exposed to DMSO and ethylene glycol. Cryoprotectant concentration and equilibration period had a significant (p lower than 0.05) effect on the total activity of the two glycolytic enzymes. In both species ethylene glycol was more toxic than DMSO. The reduction in the total activity of these enzymes was probably due to the damage to the perivitelline membrane and blastoderm caused by the osmotic stress and partial denaturation of the leached enzymes within the perivitelline space.

Details

  • Original title: Effect of cryoprotectants on activity of selected enzymes in fish embryos.
  • Record ID : 1995-3881
  • Languages: English
  • Source: Cryobiology - vol. 32 - n. 1
  • Publication date: 1995/02

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