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Cryopreservation of pre-hatch embryos of zebrafish (Brachydanio rerio).

Author(s) : ZHANG T., RAWSON D. M., MORRIS G. J.

Type of article: Article

Summary

The toxicity of five cryoprotectants: methanol, DMSO, glycerol, ethanediol and sucrose, on different development stages of zebrafish embryos was investigated. Embryos were exposed to a range of cryoprotectant concentrations for 30 min at room temperature. Post-heart beat stage embryos were more resistant to cryoprotectants than early embryonic stages. Methanol was more effective than either DMSO or ethanediol for zebrafish embryo cryopreservation and 0.3 deg C/min was found to be the optimum cooling rate. Two-step addition of cryoprotectants, with the higher concentration of cryoprotectants added at 0 deg C, improved the results.

Details

  • Original title: Cryopreservation of pre-hatch embryos of zebrafish (Brachydanio rerio).
  • Record ID : 1994-0581
  • Languages: English
  • Source: Aquat. living Resour./Ressour. vivantes aquat. - vol. 6 - n. 2
  • Publication date: 1993
  • Document available for consultation in the library of the IIR headquarters only.

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