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Effects of cryoprotectant agents and freezing protocol on motility of black-lip pearl oyster (Pinctada margaritifera L.) spermatozoa.

Author(s) : ACOSTA-SALMÓN H., JERRY D. R., SOUTHGATE P. C.

Type of article: Article

Summary

Gamete cryopreservation techniques have been applied to several bivalve mollusc species. However, research activity in this area has primarily focused on cryopreserving gametes from edible oysters (Ostreiidae). Few studies have examined the effect of cryoprotectants and freezing protocols in the preservation of spermatozoa from cultured pearl oysters (Pteriidae). Pearl oyster producers are increasingly looking towards the development of improved family lines and, as a consequence, the ability to cryopreserve gametes would bring about significant benefits to the cultured pearl industry. In response to this need, the authors evaluated the effect of three cryoprotectant additives (CPA) on motility of spermatozoa from the black-lip pearl oyster, Pinctada margaritifera. These additives have previously been used to cryopreserve gametes of other bivalve species. The following CPA mixtures were evaluated: 1) 0.45 M trehalose and 0, 0.64, 1.02 and 1.53 M dimethylsulfoxide DMSO; 2) 0.2 M glucose and 2 M DMSO; and 3) 1.31 M propylene glycol (PG). The effects of four different freezing protocols on motility of P. margaritifera spermatozoa were also evaluated (slow, medium, medium-rapid and rapid cooling). This study shows that total motility was best retained when spermatozoa were cryopreserved in 0.45 M trehalose and 0, 0.64, 1.02 or 1.53 M DMSO and frozen using slow to medium-rapid cooling rates (2.1-5.2°C/min). Rapid freezing through direct plunging of spermatozoa into liquid nitrogen resulted in the lowest overall retention of motility regardless of the CPA additive; however, CPA mixture also influenced retention of motility, with 0.2 M glucose in 2 M DMSO and 1.31 M PG retaining the lowest levels of motility for the CPAs evaluated. [Reprinted with permission from Elsevier. Copyright, 2006].

Details

  • Original title: Effects of cryoprotectant agents and freezing protocol on motility of black-lip pearl oyster (Pinctada margaritifera L.) spermatozoa.
  • Record ID : 2007-1311
  • Languages: English
  • Source: Cryobiology - vol. 54 - n. 1
  • Publication date: 2007/02

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