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CRYOPRESERVATION OF SPERM FROM THE MARINE SHRIMP SICYONIA INGENTIS.

Author(s) : ANCHORDOGUY T. J.

Type of article: Article

Summary

SPERM FROM A MARINE SHRIMP, SICYONIA INGENTIS, WERE FROZEN TO 77 K (-196 DEG C) USING A VARIETY OF COOLING RATES AND CRYOPROTECTANTS. A COOLING RATE OF 1 K/MIN RESULTED IN MINIMAL CELL BREAKAGE. SPERM SAMPLES WERE FROZENIN SOLUTIONS OF KNOWN MEMBRANE STABILIZERS: TREHALOSE, SUCROSE, PROLINE, AND GLYCEROL. THE HIGHEST SPERM SURVIVAL (56%) WAS OBTAINED WITH SAMPLES FROZEN AT 1 K/MIN IN A 5% (V/V) DMSO SOLUTION. NO DECREASE IN VIABILITY WAS SEEN IN SPERM SAMPLES STORED IN LIQUID NITROGEN AT 77 K FOR 1 MONTH.

Details

  • Original title: CRYOPRESERVATION OF SPERM FROM THE MARINE SHRIMP SICYONIA INGENTIS.
  • Record ID : 1989-0790
  • Languages: English
  • Source: Cryobiology - vol. 25 - n. 3
  • Publication date: 1988/06

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