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Germination, growth, and toxin production of nonproteolytic Clostridium botulinum as affected by multiple barriers.

Author(s) : ELLIOTT P. H., SCHAFFNER D. W.

Type of article: Article

Summary

The effect of combinations of pH (6.5, 5.75), NaCl (0.25, 1.75%), and incubation temperatures (7, 13 °C) on spore germination, outgrowth, and time to toxicity of nonproteolytic Clostridium botulinum was examined. Spores of four toxin type E and nonproteolyptic type B were inoculated (10000/ml) into Tryptone Peptone Glucose Yeast Extract (TPGY) broth. Cultures were monitored for 3 weeks, or until toxin was detected. A modified FSIS-amplified ELISA was used to screen for neurotoxin. Combinations of the most inhibitory level for each barrier reduced the degree and rate of germination, the lag and growth rate of vegetative cells, and the time to toxicity.

Details

  • Original title: Germination, growth, and toxin production of nonproteolytic Clostridium botulinum as affected by multiple barriers.
  • Record ID : 2002-2329
  • Languages: English
  • Source: Ital. J. Food Sci. - vol. 66 - n. 4
  • Publication date: 2001/05
  • Document available for consultation in the library of the IIR headquarters only.

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