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Importance of sucrose for the acquisition of tolerance to desiccation and cryopreservation of oil palm somatic embryos.

Author(s) : DUMET D., ENGELMANN F., CHABRILLANGE N., DUVAL Y., DEREUDDRE J.

Type of article: Article

Summary

The importance is underlined of preculture with high sucrose concentration for the acquisition of resistance of oil palm somatic embryos to desiccation and cryopreservation. Survival of non-pregrown control embryos decreased for dehydration periods longer than 10 hours. No survival was obtained after freezing in liquid nitrogen, whatever the desiccation duration. With pregrown control embryos, 100% survival was noted, whatever the dehydration period. Survival after freezing in liquid nitrogen was possible without dehydration (40%) but it was dramatically improved after 12 (70%) and 16 hours of desiccation (80%).

Details

  • Original title: Importance of sucrose for the acquisition of tolerance to desiccation and cryopreservation of oil palm somatic embryos.
  • Record ID : 1994-0935
  • Languages: English
  • Source: Cryo-Letters - vol. 14 - n. 4
  • Publication date: 1993/07
  • Document available for consultation in the library of the IIR headquarters only.

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