ShareImprovement of ram sperm cryopreservation protocols assessed by sperm quality parameters and heterogeneity analysis.
Author(s) : OLLERO M., PEREZ-PE R., MUIÑO-BLANCO T., CEBRIAN-PEREZ J. A.
Type of article: Article
Summary
The results showed that ram spermatozoa undergo a dramatic loss of heterogeneity, viability, motility, and positive response to the hyposmotic swelling test during freezing and thawing. These changes were partially prevented by diluting samples in Fiser's extender containing egg yolk and glycerol. A considerable increase in sperm quality with respect to that obtained in Fiser's control sample was achieved by the addition of bovine lactalbumin, vitamin E, bovine serum albumin, and bovine and ram seminal plasma.
Details
- Original title: Improvement of ram sperm cryopreservation protocols assessed by sperm quality parameters and heterogeneity analysis.
- Record ID : 1999-3907
- Languages: English
- Source: Cryobiology - vol. 37 - n. 1
- Publication date: 1998/08
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Sperm; Ram; Enhancement; Cryobiology; Process; Cryopreservation
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- Source: Reprod. Fertil. Dev. - vol. 7 - n. 1
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