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Predictive water loss curves for ram spermatozoa during cryopreservation: comparison with experimental observations.

Author(s) : DUNCAN A. E., WATSON P. F.

Type of article: Article

Summary

Calculated curves predicting intracellular water loss during cryopreservation at different cooling rates were calculated from published equations. To compute these curves, basic cell parameters specific to ram spermatozoa were measured. Several discrepancies were noted between the resulting calculated curves and experimental observations made on the effects of cooling rate on sperm cell survival. These could be due to errors in the estimates of the basic parameters, or to false assumptions in the basic equations used to compute the curves. Nevertheless, the study represents a first attempt to predict intracellular water loss from ram sperm during cooling and may provide a novel approach for studies carried out to investigate cryopreservation of sperm.

Details

  • Original title: Predictive water loss curves for ram spermatozoa during cryopreservation: comparison with experimental observations.
  • Record ID : 1993-0541
  • Languages: English
  • Source: Cryobiology - vol. 29 - n. 1
  • Publication date: 1992/02

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