Share

Isolation, hypothermic storage, vitrification and ultrarapid freezing of trophoblastic vesicles.

Author(s) : GRISHCHENKO V. I., OSTASHKO F. I., ISACHENKO V. V., ISACHENKO E. F., DAKHNO F. V., SUSHKO A. B., POLSHCHIKOV G. L.

Type of article: Article

Summary

Embryos were collected from superovulated cows on the 13th and 17th days of the sexual cycle, cut into pieces and frozen by direct plunging into liquid nitrogen. After hypothermic storage, trophoblast and embryo pieces were placed to a humid chamber of thermostat for cultivation. Vesicles developed from the trophoblast particles were subject to vitrification. The development of trophoblast pieces subject to hypothermia and vitrification was virtually as good as that of fresh trophoblast pieces.

Details

  • Original title: Isolation, hypothermic storage, vitrification and ultrarapid freezing of trophoblastic vesicles.
  • Record ID : 1994-0573
  • Languages: English
  • Source: Probl. Cryobiol. - n. 4
  • Publication date: 1992
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (5)
See the source

Indexing