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State and phase transition behaviors of Quercus rubra seed axes and cotyledonary tissues: relevance to the desiccation sensitivity and cryopreservation of recalcitrant seeds.

Author(s) : SUN W. Q.

Type of article: Article

Summary

Freezing and melting transitions of cellular water in embryonic axes and cotyledonary tissues of recalcitrant Quercus rubra (red oak) seeds were compared under slow and rapid cooling conditions. The relevance of desiccation sensitivity (critical water content) and state/phase transition behaviors to cryopreservation was examined. A complete state/phase transition diagram for red oak axes was constructed, and a vitrification-based cryopreservation protocol that employed predehydration and rapid cooling was examined. State/phase transition behaviors of cellular water are important parameters for cryopreservation; however, vitrification alone was not sufficient for seed tissues to survive the cryopreservation condition.

Details

  • Original title: State and phase transition behaviors of Quercus rubra seed axes and cotyledonary tissues: relevance to the desiccation sensitivity and cryopreservation of recalcitrant seeds.
  • Record ID : 2000-2243
  • Languages: English
  • Source: Cryobiology - vol. 38 - n. 4
  • Publication date: 1999/06

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