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Steps towards the cryopreservation of the biological control agent aphidoletes aphidimyza Rond. (Diptera, Cecidomyiidae).

Author(s) : MILES J. E., BALE J. S.

Type of article: Article

Summary

The eggs of an innately cold intolerant biological control agent, Aphidoletes aphidimyza Rond., were chemically permeabilised and successfully maintained in a culture medium. High survival of the embryos was achieved after loading and unloading of 3 M methanol and 2 M glycerol but there was no survival of cryoprotectant-loaded embryos following slow cooling protocols probably due to chilling injury. A very small number of embryos survived rapid cooling (to -196 deg C) and warming after loading with 2 M glycerol, and developed to adult; however, this result could not be repeated. It was thought that embryo age was a critical factor in obtaining survival but this variable could not be easily controlled.

Details

  • Original title: Steps towards the cryopreservation of the biological control agent aphidoletes aphidimyza Rond. (Diptera, Cecidomyiidae).
  • Record ID : 1996-3741
  • Languages: English
  • Source: Cryo-Letters - vol. 16 - n. 6
  • Publication date: 1995/11
  • Document available for consultation in the library of the IIR headquarters only.

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