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The development of juvenile plants of the hybrid orchid Bratonia after seed cryopreservation.

Author(s) : POPOV A. S., POPOVA E. V., NIKISHINA T. V., et al.

Type of article: Article

Summary

The development of juvenile plants of hybrid Bratonia orchid in vitro after seed storage in liquid nitrogen and the effect of nutrient medium composition on protocorm multiplication and plant regeneration were investigated. Cryopreservation did not inhibit the germination rate of seeds. Protocorms derived from cryopreserved seeds developed faster than protocorms from control during the first 45 days. But during further culturing, this tendency was not retained and protocorms from cryopreserved seeds were of the same size as control ones. There were no significant differences in leaf numbers and shoot lengths between juvenile plants derived from unfrozen and cryopreserved seeds. The Morel medium was the most suitable for protocorm multiplication, and the liquid MS medium with half-strength macronutrients was the best for the development of juvenile plants.

Details

  • Original title: The development of juvenile plants of the hybrid orchid Bratonia after seed cryopreservation.
  • Record ID : 2005-0202
  • Languages: English
  • Source: CryoLetters - vol. 25 - n. 3
  • Publication date: 2004/05

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